02-630-0
Mouse IgG2b Isotype Control, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 02-630-0-Each-of-1 | In Stock | ₹ 17,026.44 |
02-630-0 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,026.44
GST (18%): ₹ 3,064.759
Total Price: ₹ 20,091.199
Antigen
Mouse IgG2b
Concentration
1mg/mL
Formulation
PBS with 0.1% sodium azide; pH 7.4
Host Species
Mouse
Purification Method
Purified
Regulatory Status
RUO
Target Species
None
Form
Liquid
Applications
ELISA
Conjugate
Unconjugated
Gene Alias
IgG2, IgG2b
Immunogen
Mouse IgG2b
Quantity
1 mg
Primary or Secondary
Isotype Control (Primary)
Content And Storage
Store at -20°C, Avoid Freeze/Thaw Cycles
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Description
- The isotype of a primary antibody and the application it is being used in can result in background staining
- Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
- Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
- While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
- Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined
- If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.