Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Violet™ 480, eBioscience™, Invitrogen™

Description

• Description: The rat IgG2b kappa monoclonal antibody is useful as an isotype control immunoglobulin
• Applications Reported: This eB149/10H5 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eB149/10H5 antibody has been tested by flow cytometric analysis of normal human peripheral blood and mouse bone marrow cells
• Use at the same concentration as the experimental antibody
• Brilliant Violet™ 480 (BV480) is a dye that emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information
• Our internal testing suggests that Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation
• Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser
• BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
• Powered by Sirigen.™ Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies
• IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization
• Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.