Mouse IgG2b kappa Isotype Control (eBMG2b), FITC, eBioscience™, Invitrogen™

Description

• Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin
• Applications Reported: This mouse IgG2b isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis
• Applications Tested: FITC Mouse IgG2b Isotype Control is offered in 2 formats: - μg size: has been tested by flow cytometric analysis and can be used at the same concentration as the experimental antibody.- test size: has been pre-titrated and tested by intracellular flow cytometric analysis and can be used at test size: 5 μL (1 μg)/test per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Mouse IgG2b is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen
• It is the third most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response
• Mouse IgG2b is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity
• It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation
• Mouse IgG2b is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry
• It is also used in therapeutic applications, such as immunotherapy and vaccine development.