Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin
• Applications Reported: This eBio299Arm antibody has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis
• Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes
• Use isotype control at the same concentration as experimental antibody
• eFluor® 660 is a replacement for Alexa Fluor® 647
• eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• The isotype of a primary antibody and the application it is being used in can result in background staining
• Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
• Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
• While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
• Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined
• If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.