Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin
• Applications Reported: This rat IgG1 isotype control has been reported for use in flow cytometric analysis
• Applications Tested: Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions
• It should be used at the same concentration as the experimental antibody
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics
• These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants
• Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens
• It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens
• Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases
• With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.