Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™, Invitrogen™

Description

• Description: This is a monoclonal mouse IgG2a, kappa antibody
• It is used as an isotype control for mouse IgG2a antibodies
• Applications Reported: This mouse IgG2a isotype control has been reported for use in flow cytometric analysis
• Applications Tested: The APC conjugated mouse IgG2a isotype control is offered in 2 formats: - μg size: has been tested by flow cytometric analysis of normal human peripheral blood cells and can be used at less than or equal to 1 μg/million cells (100 μL blood)
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells and can be used at test size: 5 μL (1 μg)/test per million cells (100 μL blood)
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen
• It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response
• Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity
• It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation
• Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry
• It is also used in therapeutic applications, such as immunotherapy and vaccine development.