Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin
• Applications Reported: PerCP-eFluor® 710 Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis
• Applications Tested: PerCP-eFluor® 710 Rat IgG2b Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells.Use at the same concentration as experimental antibody
• PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5
• PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm)
• Please make sure that your instrument is capable of detecting this fluorochrome
• For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative
• Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols
• Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies
• IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization
• Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.