Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 600, eBioscience™, Invitrogen™

Description

• The isotype of a primary antibody and the application it is being used in can result in background staining
• Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
• Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
• While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
• Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined
• If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody
• Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications
• It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC)
• Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains
• It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.