7069876
Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™, Invitrogen™
Rat Isotype Control
Manufacturer: Fischer Scientific
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Antigen
Rat IgG2a kappa
Concentration
1 mg/mL
Conjugate
Functional Grade
Host Species
Rat
Target Species
Not Applicable
Gene Alias
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Primary or Secondary
Isotype Control (Primary)
Content And Storage
4° C
Clone
eBR2a
Applications
Control, Flow Cytometry, Functional Assay
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with no preservative; pH 7.2
Isotype
IgG2a κ
Purification Method
Affinity chromatography
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Description
- Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin
- Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis and ELISA
- Applications Tested: This rat IgG2a isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and can be used at the same concentration as the experimental antibody
- Storage and handling: Use in a sterile environment
- Filtration: 0.2 μm post-manufacturing filtered
- Purity: Greater than 90%, as determined by SDS-PAGE
- Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
- Aggregation: Less than 10%, as determined by HPLC
- Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications
- It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC)
- Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains
- It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.