Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin
• Applications Reported: The eBio299Arm antibody has been reported for use as an isotype control for flow cytometry
• Applications Tested: This eBio299Arm antibody has been tested by LAL assay to determine endotoxin level and by immunofluorescent staining and flow cytometric analysis of mouse splenocytes and normal human peripheral blood
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• The isotype of a primary antibody and the application it is being used in can result in background staining
• Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
• Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
• While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
• Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined
• If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.