7090142
Rat IgG1 kappa Isotype Control (eBRG1), APC, eBioscience™, Invitrogen™
Rat Isotype Control
Manufacturer: Fischer Scientific
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Antigen
Rat IgG1 kappa
Concentration
0.2 mg/mL
Conjugate
APC
Host Species
Rat
Target Species
Not Applicable
Gene Alias
Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
Primary or Secondary
Isotype Control (Primary)
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Clone
eBRG1
Applications
Control, Flow Cytometry
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Isotype
IgG1 κ
Purification Method
Affinity chromatography
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Description
- Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin
- Applications Reported: This rat IgG1 isotype control has been reported for use in flow cytometric analysis and ELISA
- Applications Tested: This rat IgG1 isotype control has been used by flow cytometric analysis of mouse splenocyte suspension, This can be used at the same concentration as the experimental antibody
- Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
- Filtration: 0.2 μm post-manufacturing filtered
- Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics
- These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants
- Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens
- It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens
- Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases
- With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.