7090595
Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 615, eBioscience™, Invitrogen™
Rat Isotype Control
Manufacturer: Fischer Scientific
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Antigen
Rat IgG2a kappa
Concentration
0.2 mg/mL
Conjugate
eFluor 615
Host Species
Rat
Target Species
Not Applicable
Gene Alias
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Primary or Secondary
Isotype Control (Primary)
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Clone
eBR2a
Applications
Control, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Isotype
IgG2a κ
Purification Method
Affinity chromatography
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Description
- Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin
- Applications Reported: Rat IgG2a Isotype Control eFluor® 615 has been reported for use in immunohistochemical and immunocytochemical staining
- Applications Tested: Rat IgG2a Isotype Control eFluor® 615 has been tested by immunocytochemistry or immunohistochemistry
- Use at the same concentration as the experimental antibody
- This product has not been validated for flow cytometric analysis
- Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75)
- A standard Alexa Fluor® 594 filter is acceptable
- Excitation: 595 nm; Emission: 615 nm
- Filtration: 0.2 μm post-manufacturing filtered
- Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications
- It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC)
- Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains
- It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.