7091301

Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 660, eBioscience™, Invitrogen™

Rat Isotype Control

Manufacturer: Fischer Scientific

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Antigen

Rat IgG2b kappa

Concentration

0.2 mg/mL

Conjugate

eFluor 660

Host Species

Rat

Target Species

Not Applicable

Gene Alias

IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2

Primary or Secondary

Isotype Control (Primary)

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Clone

eB149/10H5

Applications

Control, Flow Cytometry, Immunohistochemistry

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Isotype

IgG2b κ

Purification Method

Affinity chromatography

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Description

  • Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin
  • Applications Reported: eFluor® 660 Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis and immunohistochemical staining
  • Applications Tested: eFluor® 660 Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocytes
  • This can be used at the same concentration as the experimental antibody
  • eFluor® 660 is a replacement for Alexa Fluor® 647
  • eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm)
  • Please make sure that your instrument is capable of detecting this fluorochome
  • Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies
  • IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization
  • Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.