Mouse IgG Isotype Control, Invitrogen™

Description

• Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration
• Purity: Based on immunoelectrophoresis at an antigen concentration of 20 mg/mL, the pattern of precipitation against goat anti-mouse whole serum is the same as that against goat anti-mouse IgG, Fc fragment specific
• No precipitin line was detected against goat anti-mouse IgA, alpha chain specific or goat anti-mouse IgM, μ chain specific
• This antibody is recommended for use in blocking assays, immunohistochemical, immunocytochemical and immunofluorescent applications
• Store product at 4°C until opened
• After opening, dilute only enough material for a single day’s use.; store remainder at 4°C under sterile conditions
• Country of Origin: USA The isotype of a primary antibody and the application it is being used in can result in background staining
• Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
• Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
• While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
• Isotype controls should match with the primary antibody's species and isotype so that the level of specific staining by the primary antibody may be accurately determined
• If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.