Dendritic Cell Marker DCIR2 Monoclonal Antibody (33D1), eBioscience™, Invitrogen™

Description

• Description: The 33D1 monoclonal antibody reacts with a mouse dendritic cell (DC)-specific surface marker, DCIR2
• The nature and biological activity of DCIR2 is unknown
• DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch
• Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4
• DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii
• Applications Reported: This 33D1 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistochemical staining
• It has also been reported in complement-mediated cytotoxicity
• Applications Tested: The 33D1 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• For detection of purified 33D1 it is recommended not to use goat anti-rat due to its low affinity for 33D1
• Rather, we recommend using biotin mouse anti-rat (cat
• 13-4813)
• The nature and biological activity of DCIR2 is unknown
• DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch
• Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4
• DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.