GFP Polyclonal Antibody, Alexa Fluor™ 488, Invitrogen™

Description

• To achieve appropriate staining results on formalin-fixed, paraffin embedded tissue sections, protease Type XIV (Actinase E) pretreatment is required
• The recommended dilution and incubation for protease is 1-2 mg/mL at 37°C for 10 minutes
• Other enzyme and Heat Induced Epitope Retrieval pre-treatment methods may not be suitable for this particular antibody
• Frozen sections have been successfully stained after fixation with acetone at 4°C
• The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal
• GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms
• As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted
• GFP technology has revealed considerable new insights in the physiological activities of living cells
• GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment
• The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors
• GFP fluorescence is stable, species independent and is suitable for a variety of applications
• GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization
• Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments
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