EGFR Antibody (DH8.3) - EGFRvIII, Mutant - BSA Free, Novus Biologicals™

EGFR

Monoclonal

1 mg/mL

Western Blot 1:100 - 1:2000, Flow Cytometry 1:10 - 1:1000, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500, CyTOF-ready

Q01279

EGFR

Synthetic peptide corresponding to the junctional region of the truncated receptor LEEKKGNYVVTDHC,conjugated to keyhole limpet haemcyanin.

Primary

Clone DH8.3 of EGF R/ErbB1 antibody is specific for Truncated EGFR which is also called EGFR type III, EGFRvIII or delta-EGFR (characterized by a deletion of 267 amino acids in the extracellular domain, leading to a receptor which is unable to bind ligand yet is constitutively active). Because of its impaired internalisation and degradation, EGFRvIII is known to enhance tumourigenesis. NOTE: Clone DH8.3 DOES NOT cross-react with full length or wildtype EGFR (wtEGFR).

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunoprecipitation, CyTOF

DH8.3

Unconjugated

PBS with 0.02% sodium azide

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, EGFR variant III, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ERBB, ErbB1, ERBB1PIG61, HER1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Mouse

0.025 mL

1956

Human

IgG1