Invitrogen™ Lipofectamine™ 3000 Transfection Reagent

Description

• Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results
• This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa)
• Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers: Superior performance—our highest efficiency reagent for difficult-to-transfect cells Improved cell viability—gentle on your cells, with low toxicity Versatile—one reagent for DNA, RNA, and co-transfection Efficiently Transfect Difficult-to-Transfect Cells Lipofectamine 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types
• Successfully transfected cell types include: Fibroblast (3T3, COS-7) Myoblast (C2C12, L6 CRL-1458) Hepatocyte (HepG2, HuH7) Erythroleukemic cell (K562) Breast carcinoma (MCF7, Hs578T) Prostate carcinoma (LNCap) Lung carcinoma (A549, NCI-H460) Osteosarcoma (U-2 OS, Saos-2) Colon carcinoma (Caco2, SW480) Pancreatic carcinoma (PANC-1) Skin melanoma (SK-MEL-28) Transfect Common Cells Efficiently and Cost-Effectively For common cell types, Lipofectamine 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount
• The 1.5mL size provides up to 1500 transfection reactions (in 24-well plates)
• Achieve Superior Transfection Efficiency While Maintaining Low Toxicity Lipofectamine 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization
• A low toxicity lipid dose (0.75μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells
• Enable High Transfection Efficiency in Novel Genome Editing Applications Lipofectamine 3000 reagent is suitable for novel genome editing applications
• It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.