Epredia™ Lab Vision™ EDTA Buffer for Heat-Induced Epitope Retrieval (10X)

Description

• 10mM EDTA buffer, pH 8.0 Dilute 10-fold with distilled water before use Storage Conditions: Store at room temperature
• Stable for up to 18 months
• Contains no preservatives
• For periods longer than 3 months, store at 2-8°C Procedure: 1
• Place five-micron thick tissue sections on glass slides coated with poly L-lysine or APTES
• 2
• Deparaffinize and rehydrate sections as usual
• 3
• Place slides in a Coplin jar containing 1mM EDTA buffer, pH 8.0; cover with a vented plastic wrap and place the jar in a steamer (Black & Decker™) for 20 minutes
• 4
• Take out the jar and let the sections cool in the jar for 20 minutes at room temperature
• THIS STEP IS VERY CRITICAL AND SHOULD NOT BE AVOIDED
• 5
• Wash sections in buffer for 2 x 5 minutes
• 6
• Block endogenous peroxidase as usual
• 7
• Wash sections in buffer for 2 x 5 minutes
• 8
• Block nonspecific sites with normal serum as usual
• 9
• Place optimally diluted primary antibody on the sections (incubation time and temperature for a given set of experimental conditions should be determined by the investigator)
• 10
• Wash sections in buffer for 2 x 5 minutes
• 11
• Remaining procedure is same as routinely performed in your laboratory.