4918938
Invitrogen™ Retic Lysate IVT™ Kit
Efficiently translates high molecular weight proteins and both capped and uncapped mRNAs
Manufacturer: Fischer Scientific
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Format
Kit
Expression Mechanism
Cell-Free Expression
For Use With (Application)
In Vitro Transcription
Product Line
Ambion™
Quantity
60 Reactions
Expressed Protein Type
Water Soluble Protein
Expression System
Cell-Free
No. of Reactions
60 Reactions
Product Type
Retic Lysate
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Description
- The Retic Lysate IVT™ Kit efficiently translates in vitro-synthesized transcripts, poly(A) RNA, and total RNA, including difficult-to-translate RNAs
- The optimized lysate results in high protein yield, biological activity, and consistent performance
- A report in Nucleic Acids Research (1) found that some commercial reticulocyte lysates had poor fidelity for initiation at the appropriate AUG initiator codon
- The Retic Lysate IVT Kit was tested using Kozak's constructs and it demonstrated high fidelity for initiating at the proper AUG
- Efficiently translates a wide variety of high molecular weight proteins and both capped and uncapped mRNAs Accommodates large volume of template mRNA How it works The lysate has been optimized for translation by the addition of an ATP-regenerating system, hemin, and calf liver tRNA
- Included in the kit is an optimized Translation Mix (-Met or -Leu) for the translation of both capped and uncapped messages along with High and Low Salt buffers
- These buffers can be used to maximize protein yield and activity from difficult-to-translate sequences.