4922762
Invitrogen™ BCECF, AM (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetoxymethyl Ester)
For measuring changes in the cytosolic pH of most cells
Manufacturer: Fischer Scientific
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Content And Storage
Store in freezer -5°C to -30°C.
For Use With (Equipment)
Microplate Reader
Dye Type
pH Indicator
Detection Method
Fluorescence
Product Type
BCECF AM pH Indicator
Shipping Condition
Room Temperature
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Description
- With pK a of approximately 6.98, cell-permeant, dual-excitation ratiometric pH indicator, AM is ideal for measuring changes in cytosolic pH of most cells Intracellular pH measurements with BCECF are made by determining pH-dependent ratio of emission intensity (detected at 535nm) when dye is excited at approximately 490nm versus emission intensity when excited at its isobestic point of approximately 440nm Cell Adhesion, Cell Analysis, Cell Metabolism, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Cell-Based Ion Channel Assays, Drug Discovery and Development, Ion Channel Biology, Ionic Homeostasis and Signaling, Target and Lead Identification and Validation, pH Detection Order Info Shipping Condition: Room temperature