Invitrogen™ Vybrant™ Apoptosis Assay Kits for apoptotic and necrotic cell staining

Description

• Apoptotic cells undergo several physiological processes, including changes in cell membrane permeability and chromatin condensation, which can be distinguished using different dyes in apoptotic and necrotic cell staining protocols
• Vybrant Apoptosis Assay kits offer streptavidin biotin-annexin V, propidium iodide (PI), YO-PRO-1, and Hoechst 33342 dyes to differentiate live from apoptotic or necrotic cells, which can then be visualized using flow cytometry
• Vybrant Apoptosis Assay kits offer quick and convenient cell staining dye-based methodologies for differentiating apoptotic and necrotic versus healthy cells, which can then be visualized using flow cytometry
• The Vybrant Apoptosis Assay Kit #4 detects apoptosis via changes that occur in cell membrane permeability
• This kit contains ready-to-use solutions of both YO-PRO-1 and propidium iodide (PI) nucleic acid stains
• YO-PRO-1 stain selectively passes through the plasma membranes of apoptotic cells and labels them with moderate green fluorescence
• Necrotic cells are stained fluorescent red with PI
• The Vybrant Apoptosis Assay Kit #5, or the Chromatin Condensation/Dead Cell Apoptosis Kit with Hoechst 33342 and PI for Flow Cytometry, provides a rapid and convenient assay for apoptosis based on fluorescence detection of the compacted state of the chromatin in apoptotic cells
• This kit contains ready-to-use solutions of blue-fluorescent Hoechst 33342 dye, which stains the condensed chromatin of apoptotic cells more brightly than the chromatin of non-apoptotic cells, and red-fluorescent PI dye, which stains dead cells
• The Vybrant Apoptosis Assay Kit #6 provides a rapid and convenient assay for apoptosis, using the bright, blue-fluorescent Alexa Fluor 350 conjugate of streptavidin in conjunction with biotin-X annexin V to detect apoptotic cell populations either by flow cytometry or imaging
• In addition, the kit includes a ready-to-use solution of the fluorescent red PI nucleic acid–binding dye
• PI is impermeant to live cells and apoptotic cells, but stains dead cells with red fluorescence, binding tightly to their nucleic acid
• The kit contains an ample amount of each reagent for about 50 flow cytometric assays.