50-113-8077
Invitrogen™FungaLight™ Yeast CFDA, AM/Propidium Iodide Vitality Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-113-8077-Each-of-1 | In Stock | ₹ 54,073.92 |
50-113-8077 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 54,073.92
GST (18%): ₹ 9,733.306
Total Price: ₹ 63,807.226
Cell Type
Yeast Cells
Detection Method
Fluorescence
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer
Dye Type
Other Label(s) or Dye(s)
Form
Solid
Product Line
FungaLight™
Shipping Condition
Room Temperature
Content And Storage
Contains 3 vials of 5-CFDA (1 mg per vial), 1 vial of propidium iodide, and 1 vial of DMSO.Store in freezer (-5 to -30°C) and protect from light.
For Use With (Application)
Viability Assay
Product Type
AM/Propidium Iodide Vitality Kit
Emission
PI: 490⁄635, CFDA: 492⁄517
Format
Tube(s), Slide(s)
Quantity
200
Solubility
DMSO (Dimethylsulfoxide)
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Description
- Description The FungaLight ™ Yeast CFDA, AM / Propidium Iodide Vitality Kit combines a cell permeable esterase substrate with a membrane integrity indicator to evaluate the vitality of yeast cells by flow cytometry or microscopy
- The acetoxymethyl ester (AM) of the esterase substrate 5-carboxyfluorescein diacetate (CFDA) allows this reagent to permeant cell membranes Once inside the cell, the lipophilic blocking and diacetate groups are cleaved by nonspecific esterases, resulting in a fluorescent, charged form that leaks out of cells very slowly
- In contrast, the membrane integrity indicator, propidium iodide penetrates yeast with damaged membranes
- With an appropriate mixture of the CFDA, AM and propidium iodide stains, esterase-active yeast with intact cell membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.