Invitrogen™ SYTO™ Deep Red Nucleic Acid Stain, for live cells

Description

• SYTO Deep Red Nucleic Acid Stain is cell-permeant dye that specifically stains the nuclei of live, dead, or fixed cells
• It can be used in live-cell fluorescent imaging workflows and in fixed cell workflows, including immunocytochemistry, immunohistochemistry, and immunofluorescence experiments
• After a brief incubation with the stain, the nuclei of live, dead, or fixed cells fluoresce with a deep red/far red signal that is detectable with a Cy5/deep red standard filter set or laser configuration
• Cell-permeant dye that stains nuclei of live, dead, or fixed cells Detectable with deep red/Cy5 fluorescence filter set, with excitation/emission maxima of 652/669 nm Five times greater fluorescent signal when staining dsDNA vs RNA SYTO Deep Red Nucleic Acid Stain can be multiplexed with blue, green, orange, red, and near-IR fluorophores when compatible fluorescent filter/laser configurations are used
• The stain increases in fluorescence with increasing concentrations of dsDNA
• SYTO Deep Red Nucleic Acid Stain displays up to five times more fluorescence when incubated with dsDNA vs RNA, unlike DRAQ or other live-cell deep red nucleic acid stains
• It has a bright initial signal and excellent photo stability in a typical imaging experiment
• These properties make the stain ideal as a simple and quantitative single-step dead/fixed cell nucleus-labeling dye for use with fluorescence microscopes, fluorimeters, fluorescence microplate readers, and flow cytometers
• The stain has been successfully used on live monolayer cells and spheroid cells and as a nuclear counter-stain in immunocytochemistry.