Invitrogen™ Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit

Description

• Assay uses AmplexRed reagent (10-acetyl-3,7-dihydroxyphenoxazine) to detect hydrogen peroxide (H 2 O 2 ) or peroxidase activity in vitro
• AmplexRed reagent, in combination with horseradish peroxidase (HRP), can detect H 2 O 2 released from biological samples, including cells, or generated in enzyme-coupled reactions
• AmplexRed reagent can also be used as an ultrasensitive assay for peroxidase activity when H 2 O 2 is in excess
• In the presence of peroxidase, AmplexRed reagent reacts with H 2 O 2 in a 1:1 stoichiometry to produce the red-fluorescent oxidation product, resorufin
• Resorufin has excitation and emission maxima of approximately 571 nm and 585 nm, respectively, and because the extinction coefficient is high (58,000 ± 5000cm– 1 M– 1 ), the assay can be performed fluorometrically or spectrophotometrically
• This reaction has been used to detect as little as 10 picomoles of H 2 O 2 in a 100μL volume or 1 x 10–5U/mL of HRP
• Can detect as little as 10 picomoles of hydrogen peroxide or 10μU/mL of horseradish peroxidase activity in a 100μL assay volume Provides sufficient reagents for approximately 500 assays using reaction volumes of 100μL per assay Cell Analysis, Cell Culture, Cell Viability, Proliferation and Function, Enzyme and Protein Activity Assays, Nitro-Oxidative Stress, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis, Reactive Oxygen Species, Stem Cell Analysis, Stem Cell Labeling and Detection, Stem Cell Research Order Info Shipping Condition: Room Temperature