Invitrogen™ Alexa Fluor™ Plus 555 Phalloidin

Description

• Alexa Fluor Plus 555 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, orange/TRITC fluorescent Alexa Fluor Plus 555 dye
• For the best possible sensitivity and specificity, Alexa Fluor Plus 555 Phalloidin is synthesized to maximize the units of Alexa Fluor Plus 555 Phalloidin per length of F-actin
• Selectively stains F-actin with 3–4 times the sensitivity of Alexa Fluor 555 Phalloidin Outstanding fluorescence performance for sharp actin cytoskeleton images Excitation/emission: 555/565 nm
• Compatible with standard TRITC/RFP filter set or laser settings More sensitive and specific than antibody staining against actin Optimal for fixed and permeabilized samples Phalloidin is a bi-cyclic peptide belonging to a family of toxins originating from the deadly Amanita phalloides 'death cap' mushroom and commonly used in imaging applications to selectively label F-actin
• Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding
• Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 555 dye provides orange/TRITC fluorescence of unparalleled brightness and photostability
• Alexa Fluor Plus 555 Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations
• Its staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.