C10289
Invitrogen™ Click-iT™ AHA Alexa Fluor™ 488 Protein Synthesis HCS Assay
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | C10289-Each-of-1 | In Stock | ₹ 67,763.52 |
C10289 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 67,763.52
GST (18%): ₹ 12,197.434
Total Price: ₹ 79,960.954
Color
Blue, Green
Detection Method
Fluorescence
For Use With (Equipment)
Fluorescence Microscope, High Content Analysis Instrument
Dye Type
Alexa Fluor™ 488, Hoechst 33258
Format
96-well plate
Product Line
Alexa Fluor™, Click-iT™, Molecular Probes™
Shipping Condition
Room Temperature
Content And Storage
The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.Store at ≤-20°C, desiccate and protect from light.
For Use With (Application)
High-content Screening (HCS)
Product Type
HCS Assay
Emission
UV, Visible
Green Features
Less hazardous
Quantity
2 plates
Related Products
Description
- The Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging
- Click-iT AHA is an amino acid analog of methionine containing an azido moiety
- Similar to 35S-methionine, Click-iT AHA is fed to cultured cells and incorporated into proteins during active protein synthesis
- Detection of the incorporated amine acid utilizes a chemoselectiove ligation or 'click' reaction between an azide and an alkyne, where the azido modified protein is detected with the green-fluorescent Alexa Fluor 488 alkyne
- The Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay has been successfully tested in A549 and U-2 OS cells with a variety of reagents that inhibit protein synthesis including cycloheximide, anisomycin and puromycin in both dose response and Min/Max format
- Less hazardous Cell Analysis, Cell Metabolism, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function Order Info Shipping Condition: Room temperature