Molecular Probes™ HCS NuclearMask™ Stains

Description

• Easily identify cell nuclei in high-content screening (HCS) assays with the versatile HCS NuclearMask Blue, Red, and Deep Red stains, which survive standard formaldehyde-based fixation and detergent-based permeabilization methods and can be applied to live and fixed cells
• Because their dyes are easily discriminated from other fluorophores, the HCS NuclearMask stains can be used in multiparametric analyses
• They can also label the entire cell (i.e., cytoplasm and nucleus) and provide an accurate backdrop against which to assess the features of interest
• The versatile HCS NuclearMask Blue, HCS NuclearMask Red, and HCS NuclearMask Deep Red stains can be used to measure DNA content and perform cell demarcation with formaldehyde-fixed cells on high content imaging and analysis (HCS) platforms
• HCS NuclearMask Blue, HCS NuclearMask Red, and HCS NuclarMask Deep Red stains have excitation/emission maxima of 355/460 nm, 622/645 nm, and 638/686 nm, respectively, when bound to DNA
• HCS NuclearMask Blue stain is easily discriminated from visible- and near-IR wavelength fluorophores, HCS NuclearMask Red stain is easily discriminated from green- and even orange-fluorescent fluorophores, and HCS NuclearMask Deep Red is easily discriminated from visible wavelength fluorophores when used in multiparametric analyses
• If total cell staining is desired, HCS CellMask stains label the entire cell (cytoplasm and nucleus) and provide an accurate backdrop against which the features of interest can be assessed
• HCS CellMask stains are available in a choice of colors for multiplexing flexibility
• Sufficient volume quantities are provided to stain ten 96-well plates using an assay volume of 100 μL per well.