Thermo Scientific™ GeneBLAzer™ ADRB3 CHO-K1 DA Assay Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1473-Each-of-1 | In Stock | ₹ 1,00,276.32 |
K1473 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,00,276.32
GST (18%): ₹ 18,049.738
Total Price: ₹ 1,18,326.058
Accession Number
NM_000025.1
Cell State
Division-Arrested Cells
Detection Method
Fluorescence
Population Status
Single Cell Clone Origin
Quantity
1 Plate
Target Entry
ADRB3
System Type
GeneBLAzer™
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• GeneBLAzer™ ADRB3 CHO-K1 DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D• GeneBLAzer™ ADRB3 CHO-K1 Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe GeneBLAzer™ ADRB3 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Gene Symbol
ADRB3
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Cell Line
CHO-K1
Druggable Target
GPCR
Related Products
Description
- The GeneBLAzer™ ADRB3 CHO-K1 DA (Division Arrested) cells contain the human Adrenergic Beta 3 Receptor (ADRB3) (Accession No
- NM_000025.1) stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line
- CellSensor™ CRE-bla CHO-K1 cells (Cat
- No
- K1129) contain a beta-lactamase (bla) reporter gene under control of the Cyclic AMP Response Element (CRE)
- The ADRB3 CHO-K1 DA cells are functionally validated for Z'-factor and EC50 concentrations of Isoproterenol
- In addition, ADRB3-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time
- These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section
- DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.