Thermo Scientific™ Tango™ HRH3-blaU2OS DA Assay Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1509-Each-of-1 | In Stock | ₹ 1,00,276.32 |
K1509 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,00,276.32
GST (18%): ₹ 18,049.738
Total Price: ₹ 1,18,326.058
Accession Number
NM_007232
Cell State
Division-Arrested Cells
Cytoplasmic Tail
Tail from AVPR2 GPCR
Gene Symbol
HRH3
Product Line
Tango™
Reporter Gene
BLA (Beta-Lactamase)
Type
Division-Arrested Cells Assay Kit
System Type
Tango™
Assay Entry
Cell-based beta-arrestin recruitment
Content And Storage
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ HRH3-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D• Tango™ HRH3-bla U2OS Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ HRH3-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method
Fluorescence
Population Status
Pool of Stable Cells
Quantity
1 Plate
Target Entry
HRH3, H3
Cell Line
U2OS
Druggable Target
GPCR
Related Products
Description
- The Tango™ HRH3-bla U2OS DA (Division Arrested) cells contain the human Histamine Receptor H3 (HRH3) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line
- This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element
- The Tango™ HRH3-bla U2OS cells and the Tango™ HRH3-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of Methylhistamine
- In addition, Tango™ HRH3-bla U2OS cells have been tested for assay performance under variable conditions
- These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section
- DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.