K1538
Thermo Scientific™ CellSensor™ NFAT bla HEK 293T Cell Line
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1538-Each-of-1 | In Stock | ₹ 9,05,224.80 |
K1538 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 9,05,224.80
GST (18%): ₹ 1,62,940.464
Total Price: ₹ 10,68,165.264
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Quantity
1 Vial
System Type
CellSensor™
Cell State
Dividing Cells
Product Line
CellSensor™
Target Entry
PKC, PKC and calcium pathway
Druggable Target
Signaling Pathway
Related Products
Description
- The CellSensor™ NFAT-bla HEK 293T cell line contains a beta-lactamase reporter gene under the control of the Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into HEK 293T cells
- Fluorescence activated cell sorting (FACS) was used to isolate clones responsive to stimulation of the NFAT pathway by PMA (Phorbol 12-Myristate 13-Acetate) and Thapsagargin (Thaps)
- The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading conditions, and EC50 concentration of Thaps at fixed concentration of PMA
- The CellSensor™ NFAT-bla HEK 293T cell line responds to agonist treatment as expected from the literature and can be adapted for high-throughput screening (including coupling of receptors to the NFAT pathway) for agonists or antagonists with compound libraries
- Candidate drugs can also be tested for dose response against this cell line
- Academic and non-profit customers, please inquire for special pricing.