Thermo Scientific™ GeneBLAzer™ HCRTR2-Gα15-NFAT-blaCHO-K1 Cells
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1737-Each-of-1 | In Stock | ₹ 8,88,112.80 |
K1737 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 8,88,112.80
GST (18%): ₹ 1,59,860.304
Total Price: ₹ 10,47,973.104
Accession Number
NM_001526.2
Cell State
Dividing Cells
Detection Method
Fluorescence
Gene Symbol
HCRTR2
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Cell Line
CHO-K1
Druggable Target
GPCR
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ HCRTR2-Gα15-NFAT-blaCHO-K1 cells• GeneBLAzer™ HCRTR2-Gα15-NFAT-blaCHO-K1 Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ HCRTR2-Gα15-NFAT-blaCHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application)
FRET
Population Status
Single Cell Clone Origin
Quantity
2 x 106Cells
Target Entry
HCRTR2
System Type
GeneBLAzer™
Related Products
Description
- GeneBLAzer™ HCRTR2-Gα15-NFAT- bla CHO-K1 cells contain the human Orexin Receptor B (HCRTR2) stably integrated into the GeneBLAzer™ Gα15-NFAT- bla CHO-K1 cell line
- GeneBLAzer™ Gα15-NFAT- bla CHO-K1 cells (Cat
- No
- K1537) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Promiscuous G protein, Gα15
- The GeneBLAzer™ HCRTR2-Gα15-NFAT- bla CHO-K1 cells are functionally validated for Z' and EC 50 concentrations of Orexin B
- In addition, GeneBLAzer™ HCRTR2-Gα15-NFAT- bla CHO-K1 cells have been tested for assay performance under variable conditions
- These data are found in the Validation & Assay Performance Summary
- The HCRTR2 receptor has been detected in the brain as well as adrenal gland, gastrointestinal tract, and pancreas
- The signaling cascades used by orexin receptors appear to be varied and cell type dependant
- In CHO cells, calcium release is seen after agonist treatment
- In hypothalamic neurons, the frequency of action potentials is increased
- Administering either orexin peptide into the bloodstream of rats caused an increase in feeding behavior
- The orexin system is also thought to regulate neuroendocrine function and the sleep wake cycle
- HCRTR2 has been directly implicated in narcolepsy in animal studies
- For Academic pricing please login or contact us at discoverysciences@invitrogen.com.