K1834
Thermo Scientific™ LanthaScreen™ IGF-1R GripTite™ Cell Line
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1834-Each-of-1 | In Stock | ₹ 8,80,412.40 |
K1834 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 8,80,412.40
GST (18%): ₹ 1,58,474.232
Total Price: ₹ 10,38,886.632
Antagonists
IGF-1R Inhibitor II
Cell State
Dividing Cells
Detection Method
Fluorescence
Gene Symbol
IFG-1R
Primary Agonist
IGF-1
Quantity
1 Vial
Reporter Gene
GFP (EmGFP)
Type
Cell Line
System Type
LanthaScreen™
Assay Entry
Cell-based terbium phospho-specific activity (stable)
Content And Storage
LanthaScreen™ IGF-1R GripTite™ cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
For Use With (Application)
TR-FRET
Human Protein Class
Membrane Proteins
Product Line
LanthaScreen™
Readout
End Point
Target Entry
IGF-1R
Cell Line
293 GripTite MSR
Druggable Target
Kinases
Related Products
Description
- Description The IGF-1R/PI3K/AKT pathway mediates signals for cell growth, cell survival, transcription, translation, and glucose uptake
- IGF-1R is a receptor tyrosine kinase (RTK) that resides at the top of the pathway
- Upon growth factor binding extracellularly, this RTK undergoes auto-phosphorylation at multiple intracellular tyrosine residues (resulting in pathway activation)
- The LanthaScreen™ IGF-1R GripTite™ (HEK 293 MSR) cellular assay utilizes a human cell line that constitutively expresses IGF-1R fusion proteins with yellow fluorescent protein (YFP)
- This kinase target was introduced using lipid transfection and these cells are a clonal population isolated by FACS, using YFP fluorescence as a sorting marker and Blasticidin to maintain cells under selection
- Using this cell line, a homogenous immunoassay was developed with a time-resolved FRET (TR-FRET) readout in which the insulin-induced phosphorylation of multiple tyrosine residues on IGF-1R is detected in cell lysates using a generic terbium-labeled phosphospecific antibody (Tb-anti-pY20)
- This cell line has been validated with different stimuli/inhibitors and shows correct EC50/IC50 values
- Moreover, this assay has been optimized for performance under variable experimental conditions (including cell plating density, agonist stimulation time, DMSO tolerance and assay development time) and displays excellent statistical data (Z' > 0.6) and good signal-to-background.