Invitrogen™ PBFI, Tetraammonium Salt, cell impermeant

Description

• Although selectivity of PBFI for K + is less than that of calcium indicators such as fura-2, it is sufficient for detection of physiological concentrations of K + in presence of other monovalent cations Spectral response of PBFI upon ion binding permit excitation ratio measurements, and this indicator can be used with same optical filters and equipment used for fura-2 Label (Ex/Em): PBFI (approximately 340,380/500nm) Lyophilized product may be dissolved in distilled water or aqueous buffer for use Product is typically loaded into cells by diffusion from patch pipet for correlated fluorescence imaging and electrophysiological recording K d of PBFI for K + is strongly dependent on whether Na + is present, with value of 5.1mm in absence of Na + and 44mm in solutions with combined Na + and K + concentration of 135mm (which approximates physiological ionic strength) Although PBFI is only 1.5-fold more selective for K + than for Na + , this selectivity is often sufficient because intracellular K + concentrations are normally about 10 times higher than Na + concentrations Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Sodium and Potassium Detection Order Info Shipping Condition: Room temperature