Invitrogen™ CellEvent™ Caspase 3/7 Green Ready Flow™ Reagent

Description

• The product includes 2 reagents: the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent and SYTOX™ AADvanced™ Dead Cell Stain
• • Stable at room temperature • Convenient, ready-to-use format—no need to dilute, weigh, or pipette • Exceptionally bright green fluorescence upon binding to nucleic acids • Rapid staining of apoptotic cells without wash steps CellEvent Caspase-3/7 Green Ready Flow Reagent is a cell-permeant reagent that detects caspase 3/7 activity using flow cytometry
• Add 2 drops from the dropper bottle directly into 1 x 10 6 cells, incubate, and analyze
• CellEvent Caspase-3/7 Green Ready Flow Reagent consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye
• During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide
• Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorescent signal having absorption/emission maxima of ∼502/530 nm
• When used together with the SYTOX AADvanced Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells
• Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.