Invitrogen™ Annexin V Ready Flow Conjugates for Apoptosis Detection

Description

• Enjoy the convenience of ready-to-use Annexin V Ready Flow stand-alone conjugate stains, which offer up to 100-fold fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry
• There is no need to dilute, weigh, or pipette the conjugate stains; simply add one drop into cells, incubate, and analyze
• Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis
• The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold
• The Annexin V Ready Flow conjugates are provided as ready-to-use, direct-to-sample apoptotic cell stains
• Simply add one drop from the dropper bottle directly into 1 x 106 cells in 5 mM Ca2+-containing cell culture medium or annexin binding buffer (Cat
• Nos
• V13246 or BMS500BB), incubate, and analyze
• The traditional annexin staining protocol can be used as an alternative method by replacing the addition of 5 μL annexin conjugate with the addition of 1 drop from the Ready Flow dropper bottle
• The Annexin V Alexa Fluor 488 conjugate is excitable at 495 nm with an emission peak at 519 nm
• The Annexin V Alexa Fluor 647 conjugate is excitable at 650 nm with an emission peak at 665 nm
• The Annexin V APC conjugate is excitable at 650 nm with an emission peak at 660 nm
• The Annexin V Pacific Blue conjugate is excitable at 410 nm with an emission peak at 455 nm
• Annexin V staining to detect apoptotic cells can only be done on live cells and tissue
• If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal
• These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents
• For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.