Invitrogen™ Transferrin From Human Serum, Alexa Fluor™ 488 Conjugate

Description

• Transferrin is a monomeric serum glycoprotein (∼80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe 3+ atoms via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon
• Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin–receptor complex
• Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron
• Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish the lysosomally directed and recycling endosomal pathways
• These experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy
• We offer a biotinylated transferrin conjugate and more than 10 fluorescent versions
• Consult user Manual for solubility instructions
• Transferrin Specifications: • Label (Ex/Em): Alexa Fluor™ 488 (495/519) • Amount: 15 mg solid (contains 5 mg of transferrin conjugate) Key Applications of Labeled Transferrin Some of the many applications for labeled transferrin include: • Image transferrin receptor dynamics using FRET • Observe receptor trafficking in live cells by confocal laser-scanning microscopy • Investigate events occurring during endosomal acidification • Measure transferrin receptor binding affinity in mammals and parasites Order Info Shipping Condition: Room Temperature