Invitrogen™ Dynabeads™ Biotin Binder

Description

• Dynabeads™ Biotin Binder are 2.8 μm superparamagnetic beads that bind biotinylated antibodies (or other biotinylated ligands), allowing you to isolate cells from any species (e.g., human, mouse, rat, non-human primates, and cell lines)
• • Works with any cell type, from any sample and from any species • Fast and efficient depletion or positive isolation of any target cells for molecular downstream assays using a biotinylated primary antibody • Obtain pure and untouched cells using a mixure of biotinylated antibodies to deplete unwanted cells Adaptable for various isolation strategies The biotinylated primary antibody may be added to the cell sample (indirect technique) or pre-coated onto the Dynabeads™ Biotin Binder (direct technique)
• Regardless of which of these techniques is used, when the cell sample and the Dynabeads™ Biotin Binder are mixed, the beads bind to the target cells
• Placing the sample on a magnet separates the bead-bound cells from the rest of the sample
• One or more biotinylated antibodies may be used to either deplete the sample of unwanted cells (negative isolation) or to capture specific cells for downstream experiments (positive isolation)
• For isolation of bead-free cells (e.g., for flow cytometry), we recommend using your biotinylated antibody in combination with the CELLection™ Biotin Binder Kit (Cat
• No
• 11533D)
• Useful for many sample types Using Dynabeads™ Biotin Binder, you can isolate cells basically from any species, and from many starting samples such as whole blood, cord blood, peripheral blood mononuclear cells (PBMC), buffy coat, mouse spleen/lymph nodes, and tissue digests
• Order Info Shipping Condition: Room Temperature