Invitrogen™ Dynabeads™ CD4 Positive Isolation Kit

Description

• The Dynabeads™ CD4 Positive Isolation Kit supplies superparamagnetic beads that enable gentle isolation of high-purity CD4 + T cells from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests
• The cells are released from the beads using the DETACHaBEAD™ reagent to obtain bead- and antibody-free cells
• Advantages of the Dynabeads™ CD4 Positive Isolation Kit: • Gentle isolation of CD4 + T cells from any sample—no columns required • Bead- and antibody-free positive isolation of CD4 + cells for any downstream assay About Dynabeads™ CD4 Positive Isolation Kit The Dynabeads™ CD4 Positive Isolation Kit contains uniform, superparamagnetic beads (4.5 μm diameter) covalently coated with a monoclonal antibody specific for the CD4 membrane antigen predominantly expressed on the helper/ inducer subset of human T cells
• The kit also includes a DETACHaBEAD™ reagent that releases the Dynabeads™ from the purified CD4 + cells
• This gently magnetic isolation process gives highly viable bead- and antibody-free cells to be used in any downstream assay
• Magnetic bead-based separation offers easy handling Dynabeads™ are mixed with the sample in a tube where they bind to the target cells during a short incubation, after which the bead-bound cells are separated from unbound cells using a magnet
• The positively isolated cells are then washed, and DETACHaBEAD™ reagent is added to gently release the cells from the beads
• This magnetic bead-based positive isolation method gives both bead- and antibody-free cells
• The isolated CD4 + cells are phenotypically unaltered and suitable for any downstream application
• This fast and gentle isolation method does not require the use of columns, and helps ensure high purity and viability of the isolated CD4 + cells
• For depletion or isolation of untouched CD4 + cells, select from our range of Human CD4 + T Cell Isolation Products
• Order Info Shipping Condition: Room Temperature