Invitrogen™ Dynabeads™ Mouse Pan B (B220)

Description

• Dynabeads™ Mouse pan B (B220) are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC)
• • Rapid isolation of mouse T-cells from any sample—no columns required • Positive isolation for molecular downstream assays or efficient depletion of mouse B cells Excellent Yield, Purity, and Viability of Recovered Cells Dynabeads™ Mouse pan B (B220) are uniform, superparamagnetic beads (4.5 μm diameter) that enable easy isolation of mouse B cells from spleen or lymph nodes, although any single cell suspension may be used
• Dynabeads™ Mouse pan B beads are coated with a primary monoclonal antibody specific for the B220 membrane antigen
• B220 (also known as CD45R) is expressed on all B cells throughout development, from the early pro-B stage of B cell differentiation
• Dynabeads™ Mouse pan B binds to B cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet
• This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated B cells
• Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays Because of the powerful magnets used for separation, Dynabeads™ Mouse pan B (B220) can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable the efficient depletion of mouse B cells in about 30 minutes
• Dynabeads™ Mouse pan B will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified
• Note that viable cells will not be released from these beads, so if you want to isolate mouse B cells for cell-based applications, or you need to check your samples with flow cytometry, you should use Dynabeads™ Mouse CD43 (Untouched™ B cells) (Cat
• No
• 11422D)
• For Research Use Only
• Not for human or animal therapeutic or diagnostic use
• Order Info Shipping Condition: Room Temperature