Transforming Growth Factor-β1, human (hTGF-β1)

Description

• General description: Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.
• Specificity: Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells.Specific activity/EC50: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.
• Application: Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling.[1]
• Biochem/physiol Actions: Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells.[2]
• Quality: Purity: >95% (SDS-PAGE)Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)(Note: 1 EU corresponds to 0.1 ng)
• Sequence: Chain Length 2 x 112 AA
• Unit Definition: EC50 definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells.
• Preparation Note: Working concentration: 0.01 - 10 ng/mlRecombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.Note: Avoid repeated freezing and thawing.
• Other Notes: For life science research only. Not for use in diagnostic procedures.