Description

• General description: Bovine serum albumin (BSA) is a plasma protein with a calculated molecular weight (MW) of 66,430 Daltons (Da). The major protein in bovine serum, BSA assists in regulation of blood colloidal osmotic pressure. BSA is used in different applications such as immunochemistry, protein quantitation, and cell culture. This BSA product is specifically tested for the following properties:very low FA content / essentially fatty acid freelow globulin content / essentially globulin free
• Application: BSA is a known fatty acid (FA) carrier. Thus when BSA is used specifically in cell culture, control over specific FA′s used to culture cell lines is important, because different cell lines can be variably sensitive to particular fatty acids. Fatty acid-free BSA thus is useful for cell culture work, where the specific FA content must be stringently controlled, so that researchers can control the specific fatty acids needed for their particular cell lines. Fatty acid-free albumin also allows for optimal and maximum binding sites for the specific fatty acids that researchers wish to use in their particular cell culture experiments. Use of FA-free BSA also addresses concerns about endogenous FA′s potentially in non-FA-free BSA.
• Biochem/physiol Actions: Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
• Features and Benefits: Essentially fatty acid-freeEssentially globulin-free
• Preparation Note: Purified by a combination of heat shock and ethanol fractionation