Description

• General description: Carbenicillin is a semi-synthetic, broad-spectrum carboxypenicillin antibiotic with bactericidal and beta-lactamase resistant activity. It is a broad-spectrum antibiotic, meaning that it is effective against a wide range of bacteria, including both Gram-positive and Gram-negative bacteria. Carbenicillin is particularly useful against Pseudomonas aeruginosa, a Gram-negative bacterium that is often resistant to other antibiotics.Carbenicillin is commonly used in cell biology applications to prevent the growth of bacterial contaminants. It is also used in microbiology to select for bacteria that have been transformed with a vector harboring the gene encoding beta-lactamase, which makes them resistant to carbenicillin.
• Application: Carbenicillin disodium salt has been used: in the preparation of Luria-Bertani (LB) agar plates and media[1]as a selective agent in the culture media to prevent the growth of bacterial contaminants[2] in a study focused on the development of monoclonal antibodies[3]
• Biochem/physiol Actions: Carbenicillin acylates the penicillin-sensitive transpeptidase C-terminal domain by opening the lactam ring. This inactivation prevents the cross-linkage of peptidoglycan strands, thereby inhibiting the third and last stage of bacterial cell wall synthesis. This leads to incomplete bacterial cell wall synthesis and eventually causes cell lysis.
• Features and Benefits: Broad-spectrum antibiotic with bactericidal and beta-lactamase resistant activityEffective against a wide range of bacteria, including Pseudomonas aeruginosaCommonly used in Cell Biology and Biochemical applicationsOffers greater stablility than ampicillin
• Storage and Stability: Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
• Analysis Note: Stable at 37 °C for 3 days
• Other Notes: For additional information on our range of Biochemicals, please complete this form.