Bovine Collagen Type I

Description

• General description: In mammals, collagen type 1 is a widely expressed fibrous protein.[1] It constitutes to one-third of the total protein in humans.[2] Collagen fibril is the basic component of the tissues containing collagen. It is predominantly expressed in the extracellular matrix,[2] and is also found in arterial walls,[3] tendons, ligaments, skin, cornea, bone and dentin.[1] Collagen structure possesses a triple helix polypeptide strand.[2]
• Application: Collagen solution from bovine skin has been used as a substrate in cell adhesion assay.[4]
• Biochem/physiol Actions: Collagen type 1 is a versatile building material required for tissue elasticity and maintains the stability and strength in a variety of tissues.[1] Collagen is also present in byssus thread of invertebrates, which helps in surface attachment.[1] Defective collagen affects the biomechanical property of arterial tissue.[3]
• Components: Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. As a heterodimer composed of two a1 chains and one a2 chains, it spontaneously forms a triple helix scaffold at neutral pH and 37°C.
• Preparation Note: This product is prepared from type I bovine collagen purified and extracted from skin and contains a high monomer content. The raw collagen used to prepare this product has been isolated from a closed herd and purified with a GMP manufacturing process that includes inactivation of any possible prion or viral contamination. As supplied, it is a 6 mg/mL aqueous solution in 0.01 M HCl at a pH of 2.0.
• Other Notes: Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.