Hepatocyte Growth Factor human

Description

• General description: Recombinant, human hepatocyte growth factor (HGF) is produced from the 728 amino acid residue variant of the human pre-pro-HGF protein 6 and expressed in a mouse myeloma cell line NSO.The HGF gene is mapped to human chromosome 7q21.1.[1] The encoded protein is a mesenchymal-fibroblast derived cytokine.[2][3]
• Application: Hepatocyte Growth Factor human has been used as a growth factor cocktail to suspend rat fibrogen/thrombin cells.[4] It has also been used to study the tyrosine phosphorylation of MET (MET proto-oncogene, receptor tyrosine kinase) in myxofibrosarcoma cell line.[5]
• Biochem/physiol Actions: HGF′s major function is to induce in vitro hepatocytes proliferation.[6] HGF is also essential for cell migration and angiogenesis.[3] Itis associated with embryonic development and tissue homeostasis.[7] It is known to mediate placental trophoblast invasion, which is essential for normal fetal growth. Abnormal and defective HGF causes preeclampsia due to placental insufficiency. HGF exhibits its multifunctions through c-MET (proto-oncogene tyrosine kinase receptor).[6] HGF is known to induce human umbilical vein endothelial cell proliferation and vasculogenic mimicry migration in gastric cancer cells, thus serving as a good target for cancer therapy.[2] Upregulation of the HGF gene is observed in advanced stages of cancer,[3] such as pancreatic adenocarcinoma.[8]
• Other Notes: The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.
• Physical form: Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg of bovine serum albumin per 1μg of cytokine.
• Analysis Note: The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells.