Lipopolysaccharides from Salmonella enterica serotype typhimurium

Description

• Application: Lipopolysaccharides from Salmonella enterica serotype typhimurium has been used:in mice to elicit the secretion of pro-inflammatory cytokines[1]to induce cytokine production in monocytes[2]to elicit the activation of NF-κB pathway in mouse dendritic cells.[3]to induce inflammatory response in human colon adenocarcinoma Caco-2 cells[4]for stimulation of the catfish peripheral blood leukocytes (PBLs) to study estrogen receptor mRNA expression[5]
• Biochem/physiol Actions: LPS is a major constituent of the cell wall of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. LPS also acts as a specific mitogen for bone marrow derived B lymphocytes from mice, rabbits, chickens, cows, hamsters, and humans.
• Reconstitution: Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.
• Other Notes: To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.