TPO from rat

Description

• General description: Thrombopoietin (TPO) also known as c-Mpl ligand is an acidic glycoprotein expressed mainly in the liver, kidney, and bone marrow.[1] Recombinant rat TPO is a fully biologically active 174 amino acid polypeptide (18.7kDa), which contains the erythropoietin-like domain of the full length TPO protein.
• Application: Thrombopoietin (TPO) from rat has been used in experimental systems to determine the effect of TPO in recovery from thrombocytopenia.[2]
• Biochem/physiol Actions: Thrombopoietin (TPO) acts as a key physiologic regulator of megakaryocyte and platelet production in vivo.[3] Mutation of TPO or its receptor might lead to thrombocytopenia and megakaryocytopoiesis. In addition, TPO also influence the action of erythroid and erythromegakaryocytic progenitors in mouse embryonic yolk sac.[1] TPO binds to c-mpl receptor and regulates circulating platelet level.[1][4][5]
• Sequence: SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF
• Physical form: Lyophilized from 5 mM Sodium Phosphate, pH 7.6 + 75 mM NaCl.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.