Description

• Application: Optimized for passage of endothelial cell cultures.Trypsin inhibitor from Glycine max (soybean) was used for the following applications:Filteration and seeding of dispersed acini[1]Relative quantification of phospho-tau and immunoblotting analysis[2]Subcloning and transformation of VIL-4 and IL-4 cDNA, and protein expression and purification (trypsin inhibitor form glycine max is used as positive control).[3]
• Biochem/physiol Actions: This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.
• Other Notes: Contains chromatographically purified soybean trypsin inhibitor and iron supplemented calf serum.
• Unit Definition: One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
• Preparation Note: Prepared in Dulbecco′s Phosphate Buffered Saline without calcium and magnesium.