Retinol Binding Protein from human urine

Description

• General description: Retinol Binding Protein (RBP) is a member of lipocalin superfamily.[1] The normal concentration of RBP in serum is 40 to 50 μg/mL and has a molecular weight of 21 kDa. It has a turnover rate of 4 to 24 h.[2] RBP is produced in the liver in response to retinol binding trigger. It is also produced in kidney, sertoli cells of testis.[1] RBP contains a single globular domain and a highly conserved N-terminal region. It has eight antiparallel β sheets in the centre which binds retinol.[3] In human chromosome, the gene encoding RBP is localised at 3q23.[4]
• Application: Retinol Binding Protein from human urine has been used:as one of the protein in microinjecting mesencephalic cavity of chicken eggs to monitor embryonic cerebrospinal fluid/embryonic serum protein transfer[5]as an additive in basal medium for RNA transfection experiments[6]in the antibody-derivatized affinity pipettes preparation for the capture of affinity proteins[7]
• Biochem/physiol Actions: Inflammation induced by lipopolysaccharide (LPS) exposure caused a reduction in the level of retinol binding protein produced by the liver. [8]
• Physical form: Solution in 10 mM sodium phosphate, pH 7.4, 150 mM NaCl, and 0.05% NaN3