Gibco™ RPMI 1640 Medium, no glutamine

Description

• Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer
• RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas
• We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications
• Find the right formulation using the media selector tool
• This RPMI 1640 is modified as follows: With: Phenol Red Without: L-glutamine, HEPES The complete formulation is available
• Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins
• RPMI 1640 contains biotin, vitamin B 12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
• In addition, the vitamins inositol and choline are present in very high concentrations
• Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF)
• RPMI 1640 contains no proteins, lipids, or growth factors
• Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO 2 environment to maintain physiological pH.